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Cold Spring Harbor Protocols, 11(2023), p. pdb.top107896, 2023

DOI: 10.1101/pdb.top107896

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Selection of Antibiotic-Resistant Bacterial Strains and Identification of Genomic Alterations by Whole-Genome Sequencing: UsingStaphylococcus aureusand Oxacillin Resistance as an Example

Journal article published in 2023 by Merve S. Zeden ORCID, Angelika Gründling ORCID
This paper was not found in any repository; the policy of its publisher is unknown or unclear.
This paper was not found in any repository; the policy of its publisher is unknown or unclear.

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Abstract

Here, we discuss methods for the selection of antibiotic-resistant bacteria and the use of high-throughput whole-genome sequencing for the identification of the underlying mutations. We comment on sample requirements and the choice of specific DNA preparation methods depending on the strain used and briefly introduce a workflow we use for the selection ofStaphylococcus aureusstrains with increased oxacillin resistance and identification of genomic alterations.