Abstract The biological products use as a disease control alternative has been studied to reduce the impacts to the environment, men and animals, showing satisfactory results in postharvest. This study aimed to evaluate the biological agents effect in the control of Colletotrichum spp. and on postharvest quality of yellow passion fruit. The treatments were Trichoderma asperellum and Saccharomyces cerevisiae species at concentrations of of 0,5; 1,0; 1,5; 2,0 (g. L-1); Mancozeb fungicide (Dithane® 2 g i.a. L-1 water) and control (sterile distilled water). The fruits were immersed for 2 min in each treatment and then were drought. Five replications of three fruits were used to anthracnose severity analysis and yellow passion fruit physical-chemical quality in. The fruit inoculation was made with the deposition of Colletotrichum spp. on the surface of the fruit previously treated using holes which were made with the aid of a flamed perforator. In the research was evaluated: pH, total soluble solids and titratable acidity. Biological treatments reduced the anthracnose severity in yellow passion fruit. Fruit Post-harvest quality was not influenced by the biological control application. It is a viable alternative to postharvest management of anthracnose on yellow passion fruit under the studied conditions.