AbstractObjectiveTo assess whether coronavirus disease 2019 (COVID‐19) vaccination impacts menstrual bleeding quantity.DesignRetrospective cohort.SettingFive global regions.PopulationVaccinated and unvaccinated individuals with regular menstrual cycles using the digital fertility‐awareness application Natural Cycles°.MethodsWe used prospectively collected menstrual cycle data, multivariable longitudinal Poisson generalised estimating equation (GEE) models and multivariable multinomial logistic regression models to calculate the adjusted difference between vaccination groups. All regression models were adjusted for confounding factors.Main outcome measuresThe mean number of heavy bleeding days (fewer, no change or more) and changes in bleeding quantity (less, no change or more) at three time points (first dose, second dose and post‐exposure menses).ResultsWe included 9555 individuals (7401 vaccinated and 2154 unvaccinated). About two‐thirds of individuals reported no change in the number of heavy bleeding days, regardless of vaccination status. After adjusting for confounding factors, there were no significant differences in the number of heavy bleeding days by vaccination status. A larger proportion of vaccinated individuals experienced an increase in total bleeding quantity (34.5% unvaccinated, 38.4% vaccinated; adjusted difference 4.0%, 99.2% CI 0.7%–7.2%). This translates to an estimated 40 additional people per 1000 individuals with normal menstrual cycles who experience a greater total bleeding quantity following the first vaccine dose' suffice. Differences resolved in the cycle post‐exposure.ConclusionsA small increase in the probability of greater total bleeding quantity occurred following the first COVID‐19 vaccine dose, which resolved in the cycle after the post‐vaccination cycle. The total number of heavy bleeding days did not differ by vaccination status. Our findings can reassure the public that any changes are small and transient.