The introduction of non-native organisms into new areas may pose major threats to natural ecosystems. Therefore, continuous and careful national surveys for the interception of invasive non-native species are necessary. During the national survey of quarantine species in 2023, two new non-native ambrosia beetles, Anisandrus maiche (Kurentzov) and Xylosandrus compactus (Eichhoff), were recorded in Slovenia. Anisandrus maiche was recorded in three locations in the eastern part of Slovenia, with a total of 386 individuals. Three individuals of Xylosandrus compactus were collected in one location near the port of Koper. We discuss the possible pathways of introduction and dispersal.