Abstract The isotropic diffuse γ-ray background (IGRB) serves as a fundamental probe of the evolution of the extreme Universe. Although various astrophysical sources have been proposed as potential contributors to the IGRB, the dominant population is still under debate. γ-ray bursts (GRBs) are among candidate contributors of IGRB, although they are not as frequently discussed as blazars or starburst galaxies. Recent observations of TeV emission from GRB afterglows have provided fresh insights into this subject. This work aims to investigate the potential contribution of GRB afterglows to the IGRB under the standard afterglow model. We carefully examine the influence of each microphysical parameter of the afterglow model on this contribution, with a particular emphasis on the significant role played by the initial kinematic energy. To determine the energy and quantify the contribution of GRB afterglow to IGRBs, we utilize the observed GRB afterglow energy emissions from the Swift X-ray Telescope and Fermi Large Area Telescope instruments. Our calculations, considering the synchrotron self-Compton emission, suggest that GRB afterglows make up less than 10% of the IGRBs. To enhance the precision of our findings, it is crucial to further constrain these parameters by conducting additional ground-based observations of GRB afterglows.