The wild vegetation of the Eastern Desert is characterized by openness and comprises perennials and ephemerals. The current study investigated the relationship between the edaphic factors of the natural vegetation along El Sheikh Fadl–Ras Gharib Road, Southwest Suez Gulf, in the northern sector of the Eastern Desert. The vegetation structure of the study area is relatively simple. The surveyed plants included 93 species from 22 families (51 perennials and 42 annuals). Asteraceae, Brassicaceae, Amaranthaceae, and Fabaceae were the richest families, constituting the majority of plant species (53.76%). Therophytes were the most frequent life forms. About 83.87% of the total flora were pluriregional elements of different affinities. Most of the recorded taxa occupied the Irano-Turanian/Mediterranean/Saharo-Sindian/Sudano-Zambezian chorotypes. The application of TWINSPAN classification resulted in grouping the vegetation into three main vegetation groups (A, B, and C), representing distinct microhabitats. The CCA ordination indicates diversity in vegetation group A. Group B was highly associated with Na, Mg, CaCO3, silt, clay, and C/N. Group C showed a high correlation with sand, K, and N. The differences in wild plant life forms, richness, and diversity along the studied desert roadsides, in association with the soil differences, provide a good indication of plant biodiversity.