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American Association for the Advancement of Science, Science, 6660(381), 2023

DOI: 10.1126/science.add8673

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Structural insights into histone binding and nucleosome assembly by chromatin assembly factor-1

Journal article published in 2023 by Chao-Pei Liu ORCID, Zhenyu Yu, Jun Xiong ORCID, Jie Hu ORCID, Aoqun Song ORCID, Dongbo Ding ORCID, Cong Yu, Na Yang ORCID, Mingzhu Wang ORCID, Juan Yu ORCID, Peini Hou ORCID, Kangning Zeng, Zhenyu Li, Zhuqiang Zhang ORCID, Xinzheng Zhang ORCID and other authors.
This paper was not found in any repository, but could be made available legally by the author.
This paper was not found in any repository, but could be made available legally by the author.

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Abstract

Chromatin inheritance entails de novo nucleosome assembly after DNA replication by chromatin assembly factor-1 (CAF-1). Yet direct knowledge about CAF-1’s histone binding mode and nucleosome assembly process is lacking. In this work, we report the crystal structure of human CAF-1 in the absence of histones and the cryo–electron microscopy structure of CAF-1 in complex with histones H3 and H4. One histone H3-H4 heterodimer is bound by one CAF-1 complex mainly through the p60 subunit and the acidic domain of the p150 subunit. We also observed a dimeric CAF-1–H3-H4 supercomplex in which two H3-H4 heterodimers are poised for tetramer assembly and discovered that CAF-1 facilitates right-handed DNA wrapping of H3-H4 tetramers. These findings signify the involvement of DNA in H3-H4 tetramer formation and suggest a right-handed nucleosome precursor in chromatin replication.