Researchers have directed their attention to the application of nanotechnology in the biomedical sciences owing to their promising effects. In light of this, the present chapter is a concerted effort to highlight the emerging application of nanotechnology in the field of biomedical sciences. The first part of the chapter describes the introduction to biomedical science, followed by an introduction to the development of nanotechnology, the types and characteristics of nanosized materials, and their potential therapeutic properties. Next, the present chapter describes the application of nanotechnology in biomedical sciences. It also highlights the current issues regarding the usage of nanotechnology in biomedical science, especially safety, regulation, and toxicity. This chapter aims to provide new insights into the extraordinary application of nanotechnology in the biomedical science field for researchers, policymakers, regulators, healthcare professionals, and others involved in the nano biomedical application field.