AbstractAgricultural pests can develop behavioral resistance to insecticides by choosing to feed or oviposit on insecticide‐free hosts. As young larvae have relatively low mobility, oviposition preferences from female adults may play a critical role in shaping the evolutionary trajectory of pest populations. While oviposition avoidance of insecticide‐treated hosts was found in different agriculture pests, it remains unclear whether female adults actively choose to occupy insecticide‐free hosts. To address this question, we investigated feeding and oviposition preferences between imidacloprid‐treated and imidacloprid‐free plants in the Colorado potato beetle, Leptinotarsa decemlineata Say, a major potato pest. We performed behavioral choice assays on two strains that differed in both fecundity and insecticide resistance. We found that one strain preferred to feed on the insecticide‐free plants and that this preference is not innate. Meanwhile, the other strain chose plants for feeding and oviposition randomly. Further analyses of the moving patterns of the beetles suggested that the oviposition preference in the first strain is likely due to active learning.