The aim of this work is to develop and evaluate a multi-stage procedure model for the identification of use problems and optimization of usability using biosignal data. The concept is divided into 5 steps: 1. static analysis of data to identify use problems; 2. conducting interviews within the context of use and requirements analysis to investigate problems in more detail; 3. developing new interface concepts to implement the requirements and a prototype of an interface including dynamic visualization of data; 4. formative evaluation using an unmoderated remote usability test; 5. usability test with realistic scenarios and influencing factors in the simulation room. The concept was evaluated in the ventilation setting as an example. The procedure allowed the identification of use problems in the ventilation of patients as well as the development of suitable concepts and their evaluation to counteract use problems. To relieve users, ongoing analyses of biosignals with respect to the use problem are to be carried out. To overcome technical barriers, further development is needed in this area.