In this paper, the updated checklist of Serbian amphibians and reptiles is presented. The updated checklist of Serbian amphibians and reptiles was compiled from the literature on distribution, taxonomy and phylogeny. The checklist contains 48 autochthonous and two introduced species. Five species underwent taxonomic revisions (Triturus ivanbureschi, T. macedonicus, Pelobates balcanicus, Bufotes viridis and Mediodactylus kotschyi). Two species were added after recent taxonomic splits of species' complexes (Hyla orientalis and Anguis colchica). Presence of new (Lacerta trilineata) and introduced species (Hemidactylus turci-cus) was confirmed during recent fieldwork. There are at least eight more species that can potentially be present in Serbia. The national protection legislative should be updated according to the current checklist.