Published in
American Society for Microbiology, Microbiology Spectrum, 3(11), 2023
DOI: 10.1128/spectrum.00653-23
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Immunization with Recombinant Accessory Protein-Deficient SARS-CoV-2 Protects against Lethal Challenge and Viral Transmission
,
Jun-Gyu Park,
Kevin Chiem,
Piyush Dravid,
Anna Allué-Guardia,
Andreu Garcia-Vilanova,
Paula Pino Tamayo,
Vinay Shivanna,
Amit Kapoor,
Mark R. Walter,
James J. Kobie,
Richard K. Plemper,
Jordi B. Torrelles,
Luis Martinez-Sobrido
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Abstract
Live-attenuated vaccines (LAVs) are able to induce robust immune responses, including both humoral and cellular immunity, representing a very promising option to provide broad and long-term immunity. To develop LAVs for SARS-CoV-2, we engineered attenuated recombinant SARS-CoV-2 (rSARS-CoV-2) that simultaneously lacks the viral open reading frame 3a (ORF3a) in combination with either ORF6, ORF7a, or ORF7b (Δ3a/Δ6, Δ3a/Δ7a, and Δ3a/Δ7b, respectively) proteins.