Published in

American Scientific Publishers, Journal of Biomedical Nanotechnology, 9(20), p. 1480-1486, 2024

DOI: 10.1166/jbn.2024.3898



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The Role of miRNA-29b1 on the Hypoxia-Induced Apoptosis in Mammalian Cardiomyocytes

Journal article published in 2024 by Hailin Liu, Dingmin Juan, Kaize Wu, Ruhao Cao, Bo Dai
Distributing this paper is prohibited by the publisher
Distributing this paper is prohibited by the publisher

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A hypoxic stress which causes apoptosis of cardiomyocytes is the main problem in the ischemic heart disease. The present research sought to investigate the role of microRNA-29b1 (miR-29b1) in hypoxia-treated cardiomyocytes and its potential mechanism involved. We replicated an in vitro AC16 and H9C2 cardiomyocytes ischemia model by hypoxia (1% O2, 48 h) treatment. Cell apoptosis was evaluated by flow cytometry using Annexin V FITC-PI staining assay. Moreover, we used western blot and immunofluorescence analysis to determine the expression of Bcl-2, Bax caspase-3 and Cx43 proteins. We found that miR-29b1 protected AC16 and H9C2 cells from hypoxia-induced injury as the evidences that miR-29b1 attenuated the effects of hypoxia treatment on AC16 and H9C2 cell apoptosis after hypoxia treatment. In conclusion, these results revealed the potential cardiovascular protective effects of miR-29b1 in the process of ischemia-related myocardial injury.