Burnout is one of the most insidious challenges for healthcare professionals, and has been exacerbated by the COVID-19 pandemic. Gratitude is emerging as an intervention to reduce burnout. However, to the authors' best knowledge, no systematic review has previously been carried out to explore the impact of gratitude on burnout among healthcare professionals. The present study aimed to address this gap. A total of 95 publications were identified, of which 13 were included in the review. These studies provide preliminary evidence for the inverse association between gratitude and burnout, and the effectiveness of gratitude interventions in reducing burnout among healthcare professionals. Limitations of the current research and future directions are discussed, along with the implications for practice.