Currently, Brazil is the largest producer and exporter of soybeans in the world. Most of this cultivation is concentrated in the Cerrado region, which has soils with low boron levels. Boron performs functions that are directly linked to plant performance. The objective of this study was to evaluate the agronomic performance of soybean cultivars with and without boron fertilization. Two field experiments were carried out in the agricultural years 2018/2019 and 2019/2020. Each experiment was carried out in a randomized block design with four replications and 10 soybean cultivars (Desafio, Foco, Bonus, Maracaí, 7067, 7110, 7739, 8372, 7100, and Population). Boron fertilization was carried out at the V3 stage of the crop using ulexite (10% of boron) at a rate of 3194 kg ha−1 and 0.0 kg ha−1 of B. The application of boron to the soil increased plant height, pod insertion height, number of branches, main stem diameter, and number of pods per plant, in addition to increasing the cycle of these cultivars. The cultivars 7110, 7739 and Desafio did not statistically differ in terms of grain yield in response to boron fertilization. The cultivars Foco, Bonus, Maracaí, 7067, 8372, 7100, and Population responded favorably to this fertilization. Furthermore, genetic breeding programs incorporate advanced strategies, such as the use of boron fertilization, in order to improve the performance of the selected genotypes. Implementing boron fertilization as an integral part of breeding programs helps not only to achieve high-yielding cultivars but also to optimize key agronomic traits. This integrated approach not only boosts breeding research but also provides a solid basis for sustainable and efficient agricultural practices.