The National Center for Global Health and Medicine at J-GRID’s bases in Vietnam studies healthcareassociated infections caused by multiple-drugresistant bacteria, which have become an issue in southeast Asian countries. As a pilot study in a single facility, we retrospectively investigated blood culture status and results. From reports over the four years from 2009 to 2012, we counted 45,366 blood cultures. The sampling frequency was 9.59 blood cultures per 1,000 patient-days. Blood culture results in 2009 showed that 16 samples (1.3%) tested positive for Pseudomonas aeruginosa and 37 (3.0%) for Acinetobacter sp., two strains known to often cause medical infections. This study showed the possibility of obtaining the appropriate number of blood cultures and characteristic of isolated bacteria in Vietnam in the future.