Concerns have been raised over the release of C-60 nanoparticles into the environment and the potential risk to human health. To address these concerns it is essential to understand the pathways by which nanoparticles enter the cell, where they migrate to, and to establish whether the particles are transformed or modified within the cell. Imaging the subcellular distribution of carbon-based nanoparticles is particularly challenging. It is difficult to achieve high spatial resolution with sufficient image contrast to enable the nanoparticles to be identified within the cell. We have exposed human monocyte-derived macrophages (HMMs) to C-60 and used energy filtered transmission electron microscopy (EFTEM) to image the distribution of C-60 aggregates within intracellular compartments. We demonstrate that images recorded using low-loss electrons provide a significant improvement in contrast between the cellular material and the C-60 allowing a clear differentiation between C-60 and unstained cellular compartments and also between ordered and disordered forms of aggregated C-60. We confirm that C-60 is taken up by HMMs in vitro and is sequestered at several sites within the cell. These sites include the cytoplasm, lyscsomes, and most significantly the cell nuclei.