Published in
American Physiological Society, American Journal of Physiology - Renal Physiology, 5(320), p. F734-F747, 2021
DOI: 10.1152/ajprenal.00575.2020
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Role of KLHL3 and dietary K<sup>+</sup> in regulating KS-WNK1 expression
Journal article published in 2021 by
Mauricio Ostrosky-Frid,
María Chávez-Canales,
Jinwei Zhang 
,
Olena Andrukhova,
Eduardo R. Argaiz ,
Fernando Lerdo-De-Tejada,
Adrian Murillo-De-Ozores,
Andrea Sanchez-Navarro,
Lorena Rojas-Vega,
Norma A. Bobadilla ,
Norma Vazquez,
María Castañeda-Bueno ,
Dario R. Alessi,
Gerardo Gamba
,
Olena Andrukhova,
Eduardo R. Argaiz ,
Fernando Lerdo-De-Tejada,
Adrian Murillo-De-Ozores,
Andrea Sanchez-Navarro,
Lorena Rojas-Vega,
Norma A. Bobadilla ,
Norma Vazquez,
María Castañeda-Bueno ,
Dario R. Alessi,
Gerardo Gamba
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Abstract
In this work, we demonstrated that the kidney-specific isoform of WNK1 (KS-WNK1) in the kidney is modulated by dietary K+ and activity of the ubiquitin ligase protein Kelch-like protein 3. We analyzed the role of different amino acid residues of KS-WNK1 in its activity against the NaCl cotransporter and sensitivity to Kelch-like protein 3.