We provide information on the distribution, natural history, and conservation status of Nymphargus mixomaculatus (Guayasamin, Lehr, Rodriguez & Aguilar, 2006), a species from central Peru which is currently considered as Critically Endangered. We report four new localities and extend the altitudinal distribution by 430 m and the geographic range by 513 km in a straight line northwest of the previous, only known locality. Furthermore, based on our new data and following the criteria and categories of the International Union for Conservation of Nature, we suggest that N. mixomaculatus should be recategorized as Vulnerable.