Significance Endocytosis controls the proteome and lipidome of the plasma membrane and therefore the communication between the cell and the outside world. Because of its importance, many attempts have been made to develop tools to interfere with this process. These include genetic approaches and small chemicals. Thus far, however, the tools developed to modulate plant endocytosis either lack specificity, are irreversible, or require several days to produce an effect. Here, we generated a conditional and reversible tool to specifically interfere with endocytosis. Short-term heat treatment destabilizes an engineered adaptor complex subunit, which abolishes the function of the endocytic TPLATE complex. This causes accumulation of endocytic cargoes at the plasma membrane and provides a genetic background for future research.