The remains of equids are abundant in the Early Pleistocene faunas of Greece. “Apollonia-1” is one of the richest localities from the latest Villafranchian, providing eight skulls, mandibular remains and plenty of postcranial material during several field campaigns. This study focuses mainly on the skulls, mandibular remains and metapodials from the old and new collection described in detail. The specimens are compared with equids from several Greek and European fossiliferous localities dating from the late Villafranchian to the middle Galerian. The systematic position of Equus apolloniensis is also discussed. Based on its basicranial proportions, E. apolloniensis is considered a true Equus. A second species has also been identified recently, here referred to as Equus sp.; it is poorly represented, and it is even larger and more robust than E. apolloniensis based on a single metacarpal and third phalanges. The presence of two equid species in Apollonia 1 validates its Epivillafranchian (=latest Villafranchian) age.