The reported work demonstrates the design and realization of an integrated mid-band (sub-6 GHz) and mmWave multiple input, multiple output (MIMO) antenna for 5G handheld devices. The proposed prototype consists of the two-port MIMO configuration of the mid-band antenna placed at the top and bottom of the substrate, while the 4-port mmWave MIMO antenna is placed sideways. The MIMO configuration at the top and bottom consists of a two-element array to achieve high gain at the mid-band spectrum, while the antennas placed sideways are optimized to cover the 5G-mmWave band spectrum. The overall dimensions of the board were selected the same as the of smartphones, i.e., 151 mm × 72 mm. The mid-band antenna has an operational bandwidth of 2.73 GHz, whereas the mmWave antenna has an impedance bandwidth of 3.85 GHz with a peak gain of 5.29 and 8.57 dBi, respectively. Furthermore, the design is analyzed for the various MIMO performance parameters; it was found that the proposed antennas offer high performance in terms of envelop correlation coefficient (ECC), diversity gain (DG), mean effective gain (MEG) and channel capacity loss (CCL) within operational range. A fabricated prototype was tested and measured results show strong agreement with predicted results. Moreover, the proposed work is compared with state-of-the-art work for the same applications to demonstrate its potential for targeted application.