Automation of milking systems is linked to accurate measurement of fluctuations in milk flow during milking. To assess the fluctuations of the milk flow, the formation and movement of milk portions in the milking machine-milk pipeline system was studied. By considering the movement of a milk plug along the milk pipeline, a hydraulic model of the formation of a critical volume of milk in the milking machine manifold was compiled. In practice, the most expedient way of determining milk flow parameters may be to measure the laser fluorescent and extinction responses of moving air-milk mixture. We have implemented a new laser sensing method for measuring the flow rate and composition of milk on the basis of counting the optical response pulses received from moving dispersed components by a CCD array or a randomized fiber optic bundle. Using the developed laser sensors, the theoretical model of milk flow was tested.