Strawberry (Fragaria × ananassa Duch.) is widely grown and highly appreciated by consumers around the world for its delicious, soft, and highly nutritious fruits. Turkey is one of the most important strawberry producers in the world. Strawberry cultivation in Turkey typically involves the use of chemical fertilizers and more recently organic and organic + chemical fertilizers have been started to use in commercial production to produce healthier fruits. Therefore, in this study, we investigated the effect of organic, chemical, and organic + chemical fertilizer treatments in strawberry (cvs. ‘Albion’, ‘San Andreas’ and ‘Monterey’) fruit quality parameters including fruit color (L*, a*, b*, C and h°) parameters, soluble solids content, total acidity, fruit firmness, vitamin C, specific sugars and organic acids. Results showed that in particular fruit color parameters, soluble solid content (SSC), total acidity, fruit firmness, and vitamin C (L-Ascorbic acid) in fruits of three strawberry cultivars were significantly affected by different fertilizer applications (p < 0.05). Compared with conventional chemical fertilizer treatment, the organic fertilizer treatment produced fruit with significantly higher contents of SSC and glucose but decreased fruit firmness and vitamin C. Organic fertilizer also gave more intense colored strawberry fruits with high Chroma values (47,948 in organic fertilizer application and 39,644 and 39,931 in organic + chemical fertilizer and chemical fertilizer, respectively). Citric acid was identified to be the predominant organic acid in strawberry fruits but treatments were found insignificant on citric acid content.