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Biogas is produced in Bangladesh mostly through fixed-dome anaerobic digesters, which usually operate without any temperature controller. An experiment was conducted to monitor the seasonal temperature variation inside a fixed-dome type digester and its effect on biogas composition. A commercial-scale digester with a working volume of 350 m3 was used for this study. Three k-type thermocouple sensors were used to monitor the ambient, biogas, and slurry temperatures in real-time. The results showed that the average ambient temperature in the autumn, late autumn, and winter was 29.05, 22.90, and 17.64 °C, respectively. The average slurry temperature in the autumn (30.38 °C) was higher than in the late autumn (29.36 °C) and in the winter (25.76 °C). The highest and lowest slurry temperatures were found to be 31.11 and 24.47 °C, respectively, which indicated that the digester worked within a wide temperature range, establishing both psychrophilic and mesophilic operational conditions. Higher methane concentrations were observed in the autumn than in the late autumn and winter. The CO2 and H2S concentrations were higher in the winter than those of in the autumn and late autumn. The electricity generation in the winter was 47.85% and 45.15% lower than in the autumn and late autumn, respectively.