A psychrotolerant facultative anaerobe, strain SKBG^T, was isolated from the bottom sediments of the cold mineral spring Buxichen (Buryatia, Russia). Gram-positive non-motile cocci with a diameter of 1.75–2.5 µm were observed singly or in long chains. Cells grew in the temperature range from ̶ 5–35 °C. Growth was observed within the pH range of 7.0–9.5, with the optimum growth at pH 7.6 and at a NaCl concentration from 0–1.0 % (optimum 0.1 % (w/v)). Strain SKBG^T was a chemoorganoheterotroph that used sugars and some organic acids as substrates. The predominant fatty acids in cell walls were С_16:1ω9, С_18:1ω9, and С_{16 : 0}. The 16S rRNA gene sequence of strain SKBG^T shared high similarity (>99 %) with those of the type strains of the genus Trichococcus . Digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strain SKBG^T and Trichococcus shcherbakoviae Art^T (=DSM 107162^T=VKM B-3260^T) were 70.1 and 95.4 %, respectively. The genomic DNA G+C content of strain SKBG^T was 47.1 mol%. Compared with the type strain of T. shcherbakoviae , the new strain was characterized by a temperature optimum for growth (10 °C) significantly lower than that of T. shcherbakoviae DSM 107162^T (20–30 °C). Based on phenotypic and genomic characteristics, the isolate SKBG^T was classified as T. shcherbakoviae subsp. psychrophilus subsp. nov. The type strain is SKBG^T (=VKM B-3241^Т=JCM 33326^T).