Abstract Lightning is frequently initiated within the convective regions of thunderstorms, and so flash rates tend to follow trends in updraft speed and volume. It has been suggested that lightning production is linked to the turbulent flow generated by updrafts as turbulent eddies organize charged hydrometeors into complex charge structures. These complex charge structures consist of local regions of increased charge magnitudes between which flash-initiating electric fields may be generated. How turbulent kinematics influences lightning production, however, remains unclear. In this study, lightning flashes produced in a multicell and two supercell storms simulated using The Collaborative Model for Multiscale Atmospheric Simulation (COMMAS) were examined to identify the kinematic flow structures within which they occurred. By relating the structures of updrafts to thermals, initiated lightning flashes were expected to be located where the rate of strain and rotational flow are equal, or between updraft and eddy flow features. Results showed that the average lightning flash is initiated in kinematic flow structures dominated by vortical flow patterns, similar to those of thermals, and the structures’ kinematics are characterized by horizontal vorticity and vertical shearing. These kinematic features were common across all cases and demonstrated that where flash-initiating electric fields are generated is along the periphery of updrafts where turbulent eddies are produced. Careful consideration of flow structures near initiated flashes is consistent with those of thermals rising through a storm.