Animals kept in extensive production systems are constantly exposed to the effects of climatic aspects, which could favour thermal discomfort, decreasing productive and reproductive performances. Utilisation of Bos taurus crosses with Bos indicus animals is increasing in the tropics aimed at improving production and it is now well known how these animals respond in such environment. The objective was to characterize the morpho-physiological thermoregulation responses of weaned heifers from four different genetic groups to infer regarding the different degrees of adaptation to heat under tropical conditions Forty-eight, pasture-kept, 6-month-old, weaned heifers from four distinct genetic groups: (1) Nellore; (2) Senepol; (3) Angus x Nellore; and, (4) ¼ Brahman x ¼ Nellore x ½ Senepol (Tri-cross) were evaluated. Skin, hair coat and rectal temperatures; sweating rate; respiratory and heart rates; visual analysis of hair and hair coat colour; hair coat temperature and thickness; and length, diameter, density and number of hairs were evaluated. In addition, the temperature-humidity index, black-globe temperature and humidity index, and radiant thermal load were determined. Angus x Nellore animals presented the lowest (P=0.008) respiratory rate. Rectal temperature did differ (P=0.001) between seasons. Angus x Nellore heifers showed the greatest (P=0.001) values for the morphological measures in the evaluated months, coinciding with thermal comfort indexes above those considered comfortable for cattle. Nellore (Bos indicus), Brahman x Nellore x Senepol, and Senepol (B. taurus) present the best adaptive traits in savanna. The utilisation of B. indicus animals or adapted breed confers desirable morphological characteristics for the tropical environment.