Dissemin is shutting down on January 1st, 2025

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Elsevier, Studies in Mycology, 1(100), p. 100133-100133, 2021

DOI: 10.1016/j.simyco.2021.100133

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Comparative genomic analysis of clinicalCandida glabrataisolates identifies multiple polymorphic loci that can improve existing multilocus sequence typing strategy

This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Data provided by SHERPA/RoMEO

Abstract

Candida glabratais the second leading cause of candidemia in many countries and is one of the most concerning yeast species of nosocomial importance due to its increasing rate of antifungal drug resistance and emerging multidrug-resistant isolates. Application of multilocus sequence typing (MLST) to clinicalC. glabrataisolates revealed an association of certain sequence types (STs) with drug resistance and mortality. The currentC. glabrataMLST scheme is based on single nucleotide polymorphisms (SNPs) at six loci and is therefore relatively laborious and costly. Furthermore, only a few high-qualityC. glabratareference genomes are available, limiting rapid analysis of clinical isolates by whole genome sequencing. In this study we provide long-read based assemblies for seven additional clinical strains belonging to three different STs and use this information to simplify theC. glabrataMLST scheme. Specifically, a comparison of these genomes identified highly polymorphic loci (HPL) defined by frequent insertions and deletions (indels), two of which proved to be highly resolutive for ST. When challenged with 53 additional isolates, a combination ofTRP1(a component of the current MLST scheme) with either of the two HPL fully recapitulated ST identification. Therefore, our comparative genomic analysis identified a new typing approach combining SNPs and indels and based on only two loci, thus significantly simplifying ST identification inC. glabrata. Because typing tools are instrumental in addressing numerous clinical and biological questions, our new MLST scheme can be used for high throughput typing ofC. glabratain clinical and research settings.