AbstractThermal variation has complex effects on organisms and they respond to these effects through combined behavioral and physiological mechanisms. However, it is less clear how these traits combine in response to changes in body condition (e.g., size, hydration) and environmental factors that surround the heating process (e.g., relative humidity, start temperatures, heating rates). We tested whether these body conditions and environmental factors influence sequentially measured Voluntary Thermal Maxima (VT_max) and Critical Thermal Maxima, (CT_max) in leaf-cutting ants (Atta sexdens rubropilosa, Forel, 1908). VT_max and CT_max reacted differently to changes in body size and relative humidity, but exhibited similar responses to hydration level, start temperature, and heating rate. Strikingly, the VT_max of average-sized workers was closer to their CT_max than the VT_max of their smaller and bigger sisters, suggesting foragers maintain normal behavior at higher temperatures than sister ants that usually perform tasks within the colony. Previous experiments based on hot plate designs might overestimate ants’ CT_max. VT_max and CT_max may respond concomitantly or not to temperature rises, depending on body condition and environmental factors.