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Wiley, Proteomics, 1(4), p. 27-30, 2004

DOI: 10.1002/pmic.200300624

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Optimization of the first dimension for separation by two-dimensional gel electrophoresis of basic proteins from human brain tissue

Journal article published in 2004 by Kyla Pennington ORCID, Emma McGregor, Clare L. Beasley ORCID, Ian Everall, David Cotter ORCID, Michael J. Dunn
This paper is available in a repository.
This paper is available in a repository.

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Abstract

A major cause of poor resolution in the alkaline pH range of two-dimensional electrophoresis (2-DE) gels is unsatisfactory separation of basic proteins in the first dimension. We have compared methods for the separation of basic proteins in the isoelectric focusing dimension of human brain proteins. The combined use of anodic cup-loading and the hydroxyethyldisulphide containing solution (DeStreak) produced better resolution in both analytical and micropreparative protein loaded 2-DE gels than the other methods investigated.