AbstractThe current study describes the effects of sub-lethal concentrations and constituent compounds (citral and geranyl acetate) of Cymbopogon flexuosus essential oil (EO) on the development of Aedes aegypti. We treated eggs with 6, 18, or 30 mg L⁻¹ and larvae with 3 or 6 mg L⁻¹ of EO and its major compounds (citral and geranyl acetate). Citral and geranyl acetate were evaluated at 18, 30, and 42 mg L⁻¹ and compared with commercial growth inhibitors (diflubenzuron and methoprene). We measured larval head diameter, siphon length, and larval length. Finally, we examined concentrations of molt hormone (MH) and juvenile hormone III (JH III) using high-performance liquid chromatography coupled to mass spectrometry. All geranyl acetate concentrations decreased egg hatching, while EO altered molting among larval instars and between larvae and pupae, with an increase in the larval length (3 mg L⁻¹: 6 ± 0.0 mm; 6 mg L⁻¹: 6 ± 0.7 mm) and head width (3 mg L⁻¹: 0.8 ± 0 mm; 6 mg L⁻¹: 0.8 ± 0.0 mm) compared with the control group. We did not detect chromatographic signals of MH and JH III in larvae treated with C. flexuosus EO or their major compounds. The sub-lethal concentrations C. flexuosus EO caused a similar effect to diflubenzuron, namely decreased hormone concentrations, an extended larval period, and death.