Elsevier, Transplantation Proceedings, 6(42), p. 2138-2141
DOI: 10.1016/j.transproceed.2010.05.104
Full text: Unavailable
To mitigate hyperacute rejection, pigs have been generated with a-Gal transferase gene knockout and transgenic expression of human decay accelerating factor (hDAF), MCP, and CD59. Additionally, heme-oxygenase-1 (HO-1) has been suggested to defend endothelial cells. Sera (MS) (0%, 1%, 5% , 10%, and 15%) from Formosan macaques (Macaca cyclopis, MC) , an Old World monkey wildly populated in Taiwan, was used to test the protective in vitro, effects of hDAF or hDAF/hHO -1 on porcine aortic endothelial cells (pAEC) derived from hDAF(+), hDAF(+)/hHO-1(+), and hDAF(+)/hHO-1(-) and 1 nontransgenic pAEC. Ten percent human serum (HS) served as a positive control. When MS addition increased to 10% or 15%, all transgenic pAEC exhibited a greater survival than nontransgenic pAEC. Noticeably, 15 % MS reduced survived to 40% in nontransgenic and transgenic pAEC, respectively. These results revealed that hDAF exerted protective effects against MC complement activation. However , comparing with 10% MS and HS in pAEC of nontransgenic pigs , the survivability was higher in HS, suggesting that complement activation by MS was more toxic than that by HS. Furthermore, hDAF(+)/hHO-1(+) showed no further protection against effects of MS on transgenic pAEC. ; 附設醫院外科部 ; 醫學院附設醫院 ; 期刊論文