The problem of valid and reliable research methods in paralympic sports seems of great significance, thus the aim of this study was to evaluate the validity of the Wingate test (WAnT) in assessment of anaerobic performance in elite paracanoeists competing in 200 m races. Seven paralympic athletes (32.6 ± 7.76 years), medal winners and finalists of the Paralympic Games, World and European Championships, as well as World Cups were examined. The evaluations were conducted during a competition which was held at the paralympic distance of 200 m, and included the WAnT for upper limbs and the anaerobic threshold test (ATT). Correlations of selected indices of mechanical work and power in the WAnT, as well as anaerobic threshold (AT) power, with the time recorded during the competition were used to determine indices which affected paracanoeing performance. The results indicated strong negative correlations with time achieved in the competition between peak power and total work in the WAnT, and AT power (R = -0.796 p = 0.032; R = -0.882 p = 0.009; R = -0.900 p < 0.001, respectively). The research confirmed the validity of the WAnT for the upper limbs to assess the performance level of paracanoeists. It may be concluded that the WAnT and ATT may be used for monitoring performance during the paralympic 200 m race.