In this paper, a novel two-port dual-band multiple-input-multiple-output (MIMO) antenna with enhanced isolation and high gain is presented. The presented antenna is composed of a symmetrical ground element and two identical antenna radiating elements. At the bottom of the substrate, the ground part is applied to strengthen the isolation performance of the designed MIMO antenna. The measured −10 dB bandwidth according to the input reflection coefficient S11 are 650 MHz (2.25–2.9 GHz) and 980 MHz (5.05–6.03 GHz), which are in strong agreement with the 2.4 GHz frequency band (2.4–2.4835 GHz) and the 5 GHz frequency band (5.15–5.85 GHz) of wireless local area network (WLAN) applications. The measured S21 at both the lower and the higher frequency operation bands are less than −19.3 dB. In the operating frequency bands, the measured gain is from 1.5 dBi to 3.8 dBi. The measured results show that the presented MIMO antenna is a good candidate for WLAN applications.