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American Society for Microbiology, Microbiology Spectrum, 2(10), 2022

DOI: 10.1128/spectrum.02434-21

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Reference-Grade Genome and Large Linear Plasmid of Streptomyces rimosus: Pushing the Limits of Nanopore Sequencing

Journal article published in 2022 by Lucija Slemc, Jernej Jakše ORCID, Alessandro Filisetti, Damir Baranasic, Antonio Rodríguez-García, Francesco Del Carratore ORCID, Stefano Maria Marino ORCID, Jurica Zucko, Antonio Starcevic, Martin Šala, Mercedes Pérez-Bonilla, Marina Sánchez-Hidalgo ORCID, Ignacio González ORCID, Fernando Reyes, Olga Genilloud and other authors.
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

The genomes of Streptomyces species are difficult to assemble due to long repeats, extrachromosomal elements (giant linear plasmids [GLPs]), rearrangements, and high GC content. To improve the quality of the S. rimosus ATCC 10970 genome, producer of oxytetracycline, we validated the assembly of GLPs by applying a new approach to combine pulsed-field gel electrophoresis separation and GLP isolation and sequenced the isolated GLP with Oxford Nanopore technology.