Although data currently exists pertaining to the intensity in the women’s football match, the knowledge about training is still scarce. Therefore, the aim of this study was to quantify external (locomotor activity) and internal (psychophysiological) intensities, as well as the wellness profile of the typical microcycle from professional female soccer players during the 2019/20 in-season. Ten players (24.6 ± 2.3 years) from an elite Portuguese women soccer team participated in this study. All variables were collected in 87 training session and 15 matches for analysis from the 2019–2020 in-season. Global positioning variables such total distance, high-speed running, acceleration, deceleration and player load were recorded as intensity while Rated Perceived Exertion (RPE) and session-RPE were recorded as internal measures. The Hooper Index (HI) was collected as a wellness parameter. The results showed that internal and external intensity measures were greater in matches compared to trainings during the week (match day minus [MD-], MD-5, MD-4, MD-2), p < 0.05 with very large effect size (ES). In the same line, higher internal and external intensity values were found in the beginning of the week while the lowest values were found in MD-2 (p < 0.05, with very large ES). Regarding wellness, there was no significant differences in the HI parameters between the training days and match days (p > 0.05). This study confirmed the highest intensity values during MD and the lowest on the training session before the MD (MD-2). Moreover, higher training intensities were found in the beginning of the training week sessions which were then reduced when the MD came close. Wellness parameters showed no variation when compared to intensity measures. This study confirmed the hypothesis regarding internal and external intensity but not regarding wellness.