Global food security is under-challenged due to over increasing human population, limited cropland, and risk of climate change. Therefore, an appropriate agricultural policy framework needs to be developed for food security that should be sustainable economically and ecologically. Nitrogen (N) is a crucial element that controls the growth productivity of crop plants. N accounts for around 78 volume per cent of the atmosphere but all crop plants cannot use it directly. Agricultural land is mostly dominated by cereals (e.g. rice, wheat, maize) which have specifically high N demand as compared to food legumes. Soybean exemplifies the most significant and cultivated food legume, presently cultivated worldwide under varying climatic conditions. It plays a significant role in global food security as well as agricultural sustainability due to a high seed protein and oil concentration, and low reliance on N fertilization. Soybean enriches soil health by fixing atmospheric N through biological nitrogen fixation (BNF), the most productive and economical system for N fixation and crop production, associated with more intensive production systems. However, the efficiency of BNF depends on several factors. This study is focused to develop more reliable guidelines for managing BNF by using the potential of natural agro-ecosystems.