The aim of this study was to examine differences in achievement motivation (measured with the Objective Achievement Motivation Test, OLMT, Schuhfried®) and competitiveness between male and female semi-professional football players. The OLMT objectively assessed three constructs regarding achievement motivation: motivation through personal goals, aspiration level, and motivation through competition. In addition, competitiveness was measured with the self-reported Competitiveness-10 Questionnaire. Finally, participants’ performance was assessed by three expert observers in each of ten matches. Thirty-eight football players (men = 27; women = 11) participated in the present study, and no significant differences were found in the Levene test when comparing men and women with respect to the scores obtained in the different measures used in our research. Significant differences were found in the motivation through competition (p = 0.021) as well as in self-reported competitiveness (p = 0.020) as a function of gender, with males showing higher values in both cases. No gender differences were found in aspiration level (p = 0.283) or motivation through personal goals (p = 0.897). Moreover, age and player performance did not modulate gender differences on any measures. No significant correlation was found between motivational measures and performance. In conclusion, it should be noted that the only variable on which gender differences emerged was the level of competitiveness, such that males scored higher than females on both objective and self-reported measures.