The report documents the development of a program of quality control (QC) and quality assurance (QA) for the EPA Level 1 environmental assessment health effects bioassays to ensure that all data collected are of known and documented quality sufficient to meet their intended use. It is a companion document for IERL-RTP Procedures Manual: Level 1 Environmental Assessment, Biological Tests (EPA-600-8-82-024; NTIS PB 82-228966), October 1981. This QC/QA manual presents information on the use of laboratory performance audit samples, documentation requirements for sample identification and handling, and quality control procedures and documentation requirements for the Level 1 health effects bioassays. The procedures and sample documentation forms in this document are to serve only as models for QC/QG procedures. They should not be viewed as the only acceptable approach to QC/QA.