AbstractPredator-prey interactions are one of the central themes in ecology due to their importance as a key mechanism in structuring biotic communities. In the predator-prey systems, the behaviours of persecution and avoidance impact on the ecosystem dynamics as much as the trophic interactions. We aimed to analyse the spatiotemporal co-occurrences between prey and predators in a community of medium- and large-sized mammals in southern Mexico. We predict prey will avoid sites where a predator previously passed. Contrarily, we expect a search behaviour by predators and a synchronization in activity patterns among them. We found prey does not occur either in time or space where predators have passed, suggesting an avoidance behaviour. Contrary to our expectations, we did not find significant search behaviours from predators to prey. Synchronization in the daily temporal overlap between predators was higher (Δ = 0.77–0.82) than with their prey (Δ = 0.43 – 0.81). The results suggest prey perceives the risk of predation and displays avoidance behaviour both spatially and temporally, which is consistent with the fear theory. This study provides a complementary approach to understanding the behaviour mechanism between predators and prey through camera-trapping or similar data of spatiotemporal co-occurrences.