-The development of high-quality chemical vapor-deposited mono- and few-layer MoS₂ is of high relevance for future applications in functional devices. Consequently, a detailed understanding of the growth mode and the parameters affecting it is important. Here, we show for the case of mono- and few-layer MoS₂ grown on Muscovite mica, how strain and temperature impact the growth mode. We show how misleading the determination of the number of MoS₂ layers is, solely based on Raman spectroscopy due to the occurrence of strain and changes in the growth mode. A combination of atomic force microscopy, Raman spectroscopy, and ab initio calculations reveal that that the growth at 500 °C synthesis temperature exhibits a strained layer-by-layer growth of up to three mono-layers, whereas at 700 °C, a strain release occurs and layer-by-layer growth is confined to the first mono-layer only. We relate the occurrence of strain to the formation of gas bubbles below the MoS₂ film, escaping the mica sheets during high temperature synthesis. Our analysis shows that mica substrates can be used to study strain in 2D materials without the need to apply external stress and that a detailed knowledge of the MoS₂ morphology is necessary to correctly interpret the Raman results.