BACKGROUND: Acute heart failure is a life-threatening medical condition. Thus, effective therapy is very important for this case. Utilization of standard therapy and adjunctive isosorbide dinitrate (ISDN) pump play an important role in reducing mortality, length of hospitalization, and national early warning score – NEWS 2. However, the research on the utilization of the ISDN pump as adjunctive therapy is still limited. AIM: This study aimed to analyze the association between utilization of standard therapy and adjunctive ISDN pump with clinical outcomes (mortality, length of hospitalization, and NEWS 2) in patients with acute heart failure. METHODS: This was a cohort retrospective observational study. The purposive sampling technique was utilized to select the acute heart failure patients in UNS Sukoharjo Hospital. All the data were obtained from medical records. Logistic regression was used to analyze the data. RESULTS: A total of 94 patients were included as the samples. There was a significant association between the utilization of standard therapy (OR=7.9; CI 95%= 3.1–20.4; p < 0.001) or ISDN pump (OR=0.3; CI 95%= 0.1–0.7; p < 0.001) with the length of hospitalization. However, there was no significant association between the utilization of standard therapy (OR=1.1; CI 95%= 0.2–6.6; p = 0.9) and ISDN pump (OR=0.2; CI 95%= 0.02–1.6; p = 0.1) with NEWS 2 on patients with acute heart failure. CONCLUSION: There was a significant association between the utilization of standard therapy and ISDN pump with the length of hospitalization.