American Society for Microbiology, Microbiology Spectrum, 4(10), 2022
DOI: 10.1128/spectrum.01009-22
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In this work, we describe hypervariable-locus melting typing (HLMT), a novel fast approach to pathogen typing using the high-resolution melting (HRM) assay. The method includes a novel approach for gene target selection, primer design, and HRM data analysis.