Published in
American Society for Microbiology, Microbiology Spectrum, 4(10), 2022
DOI: 10.1128/spectrum.01009-22
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Hypervariable-Locus Melting Typing: a Novel Approach for More Effective High-Resolution Melting-Based Typing, Suitable for Large Microbiological Surveillance Programs
Journal article published in 2022 by
Matteo Perini 
,
Aurora Piazza ,
Simona Panelli,
Stella Papaleo,
Alessandro Alvaro,
Francesca Vailati,
Marta Corbella,
Francesca Saluzzo ,
Floriana Gona,
Daniele Castelli,
Claudio Farina,
Piero Marone,
Daniela Maria Cirillo ,
Annalisa Cavallero,
Gian Vincenzo Zuccotti
and other authors.
,
Aurora Piazza ,
Simona Panelli,
Stella Papaleo,
Alessandro Alvaro,
Francesca Vailati,
Marta Corbella,
Francesca Saluzzo ,
Floriana Gona,
Daniele Castelli,
Claudio Farina,
Piero Marone,
Daniela Maria Cirillo ,
Annalisa Cavallero,
Gian Vincenzo Zuccotti
and other authors.
This paper is made freely available by the publisher.
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Abstract
In this work, we describe hypervariable-locus melting typing (HLMT), a novel fast approach to pathogen typing using the high-resolution melting (HRM) assay. The method includes a novel approach for gene target selection, primer design, and HRM data analysis.