Background: Canadians with disabilities make up nearly a quarter of the population yet faced barriers in accessing information about COVID-19 testing accessibility across the country. Objective: This study aimed to understand the availability of accessibility information for COVID-19 testing sites in Canada. To date, no known studies have evaluated the availability of accessibility information online.Methods: An environmental scan of COVID-19 testing websites was conducted (1) nationally through the provincial/territorial websites for COVID-19 testing and (2) in Ontario, where there was individual location information. Data on key accessibility features were extracted from the website to simulate the user experience of booking a COVID-19 test during March 2021. Results: All provinces and territories provided minimal accessibility information on their provincial or territorial COVID-19 testing websites. Only Ontario included accessibility information for individual testing locations. Out of 170 individual testing locations in Ontario, few had information about accessibility, with only 8.2% listing at least 3 of the 5 key accessibility features measured on their websites.Conclusions: This study demonstrates that, more than a year into the pandemic, there existed a clear lack of accessibility information for testing locations for Canadians with disabilities.