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American Society for Microbiology, Microbiology Spectrum, 5(10), 2022

DOI: 10.1128/spectrum.01034-22

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Qualification of a Biolayer Interferometry Assay to Support AZD7442 Resistance Monitoring

Journal article published in 2022 by Tyler Brady, Tianhui Zhang, Kevin M. Tuffy, Nantaporn Haskins, Qun Du, Jia Lin, Gilad Kaplan, Steven Novick, Tiffany L. Roe, Kuishu Ren, Kim Rosenthal, Patrick M. McTamney, Michael E. Abram ORCID, Katie Streicher, Elizabeth J. Kelly ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

SARS-CoV-2 variants harbor multiple substitutions in their spike trimers, potentially leading to breakthrough infections and clinical resistance to immune therapies. For this reason, a BLI assay was developed and qualified to evaluate the reliability of screening SARS-CoV-2 spike trimer variants against anti-SARS-CoV-2 monoclonal antibodies (MAbs) tixagevimab and cilgavimab, the components of AZD7442, prior to in vitro pseudovirus neutralization susceptibility verification testing.