In this paper, a new approach to excite sharp asymmetric resonances using a single completely symmetric split-ring resonator (SRR) inside a rectangular waveguide is proposed. The method is based on an asymmetry in the excitation of a symmetric split-ring resonator by placing it away from the center of the waveguide along its horizontal axis. In turn, a prominent asymmetric resonance was observed in the transmission amplitude of both the simulated results and the measured data. Using a single symmetric SRR with an asymmetric distance of 6 mm from the center of a rectangular waveguide led to the excitation of a sharp resonance with a Q-factor of 314 at 6.9 GHz. More importantly, a parametric study simulating different overlayer analytes with various refractive indices revealed a wavelength sensitivity of 579,710 nm/RIU for 150 μm analyte thickness.