Tuberculosis is curable, still the world’s second inflectional murderous disease, and ranked 13th (in 2020) by the World Health Organization on the list of leading death causes. One of the reasons for its fatality is the unavailability of modern technology and human experts for early detection. This study represents a precise and reliable machine vision-based approach for Tuberculosis detection in the lung through Symmetry CT scan images. TB spreads irregularly, which means it might not affect both lungs equally, and it might affect only some part of the lung. That’s why regions of interest (ROI’s) from TB infected and normal CT scan images of lungs were selected after pre-processing i.e., selection/cropping, grayscale image conversion, and filtration, Statistical texture features were extracted, and 30 optimized features using F (Fisher) + PA (probability of error + average correlation) + MI (mutual information) were selected for final optimization and only 6 most optimized features were selected. Several supervised learning classifiers were used to classify between normal and infected TB images. Artificial Neural Network (ANN: n class) based classifier Multi-Layer Perceptron (MLP) showed comparatively better and probably best accuracy of 99% with execution time of less than a second, followed by Random Forest 98.83%, J48 98.67%, Log it Boost 98%, AdaBoostM1 97.16% and Bayes Net 96.83%.