Abstract. We present regional-scale mass balances for 25 drainage basins of the Antarctic Ice Sheet (AIS) from satellite observations of the Gravity and Climate Experiment (GRACE) for time period January 2003 to September 2012. Satellite gravimetry estimates of the AIS mass balance are strongly influenced by mass movement in the Earth interior caused by ice advance and retreat during the last glacial cycle. Here, we develop an improved glacial-isostatic adjustment (GIA) estimate for Antarctica using newly available GPS uplift rates, allowing us to more accurately separate GIA-induced trends in the GRACE gravity fields from those caused by current imbalances of the AIS. Our revised GIA estimate is considerably lower than previous predictions, yielding an estimate of apparent mass change of 53 ± 18 Gt yr−1. Therefore, our AIS mass balance of −114 ± 23 Gt yr−1 is less negative than previous GRACE estimates. The northern Antarctic Peninsula and the Amundsen Sea sector exhibit the largest mass loss (−26 ± 3 Gt yr−1 and −127 ± 7 Gt yr−1, respectively). In contrast, East Antarctica exhibits a slightly positive mass balance (26 ± 13 Gt yr−1), which is, however, mostly the consequence of compensating mass anomalies in Dronning Maud and Enderby Land (positive) and Wilkes and George V Land (negative) due to interannual accumulation variations. In total, 6% of the area constitutes about half the AIS imbalance, contributing 151 ± 7 Gt yr−1 (ca. 0.4 mm yr−1) to global mean sea-level change. Most of this imbalance is caused by ice-dynamic speed-up expected to prevail in the near future.