Rattlesnake hemoglobins: Functional properties and tetrameric stability

Lombardi, Fabio Renato; Anazetti, Maristella Conte; Santos, Giovanni César; Olivieri, Johnny Rizzieri; de Azevedo, Walter Filgueira; Bonilla-Rodriguez, Gustavo Orlando

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Rattlesnake hemoglobins: Functional properties and tetrameric stability

Journal article published in 2006 by Fabio Renato Lombardi, Maristella Conte Anazetti, Giovanni César Santos, Johnny Rizzieri Olivieri, Walter Filgueira de Azevedo, Gustavo Orlando Bonilla-Rodriguez

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Abstract

The present work analyzed the tetrameric stability of the hemoglobins from the rattlesnake C. durissus terrificus using analytical gel filtration chromatography, SAXS and osmotic stress. We show that the dissociation mechanism proposed for L. miliaris hemoglobin does not apply for these hemoglobins, which constitute stable tetramers even at low concentrations.